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Refseq consultant genomes: This database is made up of NCBI RefSeq Reference and Agent genomes throughout wide taxonomy groups like eukaryotes, bacteria, archaea, viruses and viroids. These genomes are among the highest quality genomes obtainable at NCBI.
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Permit primer to amplify mRNA splice variants (demands refseq mRNA sequence as PCR template enter) Enable If enabled, this program won't exclude the primer pairs that will amplify one or more mRNA splice variants through the exact gene as your PCR template, Therefore earning primers gene-particular rather then transcript-particular (Note that it is NOT intended to generate primers which will amplify all variants.
Make it easier to can elect to exclude sequences in the chosen database from specificity examining if You're not worried about these.
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